Bionano Sample Prep Kits: Isolation & Labeling
Bionano Prep Kits™ provide the critical reagents and protocols needed to extract and label high molecular weight (HMW) DNA for use on the Irys® and Saphyr™ systems. Bionano kits are optimized for performing Bionano optical genome mapping applications on a variety of sample types.
HIGH MOLECULAR WEIGHT DNA EXTRACTION
Bionano optical genome mapping begins with the isolation of HMW DNA
Bionano sample kits are optimized for isolating and purifying HMW DNA in a process that is gentler than existing HMW DNA extraction methods. The resulting purified DNA, several megabases in length, is optimal for use with Bionano systems. Each Bionano Prep Kit allows you to perform 5-10 HMW DNA preps. Bionano kits and protocols enable the extraction of HMW DNA from a variety of sample types including soft or fibrous:
- Animal tissue
- Plant tissue
- Cell lines
- Human blood
- Bone Marrow Aspirate (BMA) – NEW!
BIONANO SAMPLE PREP KITS ORDERING INFORMATION
| NEW! 90057 |
Bone Marrow Aspirate(BMA) DNA Isolation kit – 10 reactions | For use with heparin-collected bone marrow aspirates (BMA). Compatible with Bionano DLS DNA Labeling Kit only. First-time users must order Bionano Prep SP Magnetic Retriever (#80031). |
| NEW! 80038 |
SP Tissue and Tumor DNA Isolation kit – 10 reactions | For use with human or animal tissue or tumor. Compatible with Bionano DLS DNA Labeling Kit only. First-time users must order Bionano Prep SP Magnetic Retriever (#80031). |
| 80030 | SP Blood & Cell Culture DNA Isolation Kit – 10 reactions | For use with EDTA-collected blood and mammalian cell cultures. Compatible with Bionano DLS DNA Labeling Kit only. First-time users must order Bionano Prep SP Magnetic Retriever (#80031). |
| 80002 | Animal Tissue DNA Isolation Kit – 10 reactions | For use with soft or fibrous animal tissue. Order with Bionano DLS DNA Labeling Kit or with Bionano NLRS DNA Labeling Kit. |
| 80004 | Blood and Cell Culture DNA Isolation Kit – 10 reactions | For use with blood and cell cultures. Order with Bionano DLS DNA Labeling Kit or with Bionano NLRS DNA Labeling Kit. |
| 80003 | Plant DNA Isolation Kit – 5 reactions | For use with most plant tissues. Order with Bionano DLS DNA Labeling Kit or with Bionano NLRS DNA Labeling Kit. |
| 80005 | DLS DNA Labeling Kit – 10 reactions | Direct Label and Stain Kit for use with Bionano Animal Tissue, Plant, or Blood and Cell Culture DNA Isolation Kits. Not required for using the Bionano Prep Kit. |
| 80001 | NLRS DNA Labeling Kit – 10 reactions | Nicking, Labeling, Repairing and Staining Kit for use with Bionano Animal Tissue, Plant, or Blood and Cell Culture DNA Isolation Kits. Not required for using the Bionano Prep Kit. |
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LABELING REAGENTS DEVELOPED SPECIFICALLY FOR BIONANO SYSTEMS
Bionano labeling reagents are optimized for applications on Bionano optical genome mapping systems
Starting with HMW DNA purified using the appropriate Bionano prep kit, fluorescent labels are attached to specific sequence motifs. The result is uniquely identifiable genome-specific label patterns that enable de novo map assembly, anchoring sequencing contigs and discovery of structural variations starting at 500 bp.